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ion plus fragment library kit 48 reactions  (Thermo Fisher)


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    Thermo Fisher ion plus fragment library kit 48 reactions
    Ion Plus Fragment Library Kit 48 Reactions, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ion+plus+fragment+library+kit+48+reactions/ion+plus+fragment+library+kit+48+rxns/pm40646827-105-4-11
    Average 90 stars, based on 1 article reviews
    ion plus fragment library kit 48 reactions - by Bioz Stars, 2026-09
    90/100 stars

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    Sequencing:

    Article Title: The impact of combined thymol and rosmarinic acid on the intestinal microbiota and barrier function of the piglets challenged by Escherichia coli K88.
    Article Snippet: .. To generate sequencing libraries, the purified amplicons were processed using Illumina's Ion Plus Fragment Library Kit 48 reactions (Thermo Fisher Scientific). .. Following standard protocols, the prepared libraries were sequenced on the MiSeq platform by Illumina.

    Article Title: Effects of Coumarin on Rhizosphere Microbiome and Metabolome of Lolium multiflorum
    Article Snippet: The purification of the PCR product was performed using a GeneJET gel kit (Thermo Fisher Scientific, Waltham, MA, USA). .. The Ion Plus Fragment Library Kit 48 reactions (Thermo Fisher Scientific, Waltham, MA, USA) was used to construct the library, and sequencing was performed on an Ion S5TMXL platform. ..

    Article Title: Multiomics Reveals Alterations in the Gut Microbiome, Host Proteins, and Host Metabolites Correlating with SADS-CoV Pathogenicity and the Immune Response in Piglets
    Article Snippet: The PCR products were then mixed in equidensity ratios and puri ed using the GeneJETTM Gel Extraction Kit (Thermo Scienti c). .. Sequencing libraries were prepared using the Ion Plus Fragment Library Kit 48 reactions (Thermo Scienti c) according to the manufacturer's instructions. .. Library quality was assessed using a Qubit® 2.0 Fluorometer (Thermo Scienti c).

    Article Title: Insights into freshwater ciliate diversity through high throughput DNA metabarcoding
    Article Snippet: All PCR reactions were carried out with Phusion ® High-Fidelity PCR Master Mix (New England Biolabs). .. The mixed PCR products were purified, and sequencing libraries were generated using Ion Plus Fragment Library Kit 48 reactions (Thermo Scientific) following the manufacturer's protocol. ..

    Article Title: Porcine intestinal antimicrobial peptide as an in-feed antibiotic alternative improves intestinal digestion and immunity by shaping the gut microbiota in weaned piglets.
    Article Snippet: Antibiotic resistance of pathogens, which is caused by the abuse of in-feed antibiotics, threatens the sustainable development of livestock production.. The present study aimed to investigate the efficiency of porcine intestinal antimicrobial peptide (PIAP) as an alternative to in-feed antibiotics in terms of growth performance, intestinal morphology, digestive enzymes and immunity, and microbiota community of the post-weaning piglets.. A total of 204 piglets (Duroc Landrace Yorkshire, weaned at 28 d age) with a similar body weight of 7.97 ± 1.04 kg were randomly allocated to 4 groups (51 piglets per group): (1) control group: basal diet; (2) AB group: antibiotic, basal diet þ chlortetracycline (1000 mg/kg from d 1 to 24; 500 mg/kg from d 25 to 37); (3) P1 group: basal diet þ a relatively low dose of PIAP (400 mg/kg from d 1 to 24; 300 mg/kg from d 25 to 37); (4) P2 group, basal diet þ a relatively high dose of PIAP (600 mg/kg from d 1 to 24; 500 mg/kg from d 25 to 37).

    Purification:

    Article Title: The impact of combined thymol and rosmarinic acid on the intestinal microbiota and barrier function of the piglets challenged by Escherichia coli K88.
    Article Snippet: .. To generate sequencing libraries, the purified amplicons were processed using Illumina's Ion Plus Fragment Library Kit 48 reactions (Thermo Fisher Scientific). .. Following standard protocols, the prepared libraries were sequenced on the MiSeq platform by Illumina.

    Article Title: Insights into freshwater ciliate diversity through high throughput DNA metabarcoding
    Article Snippet: All PCR reactions were carried out with Phusion ® High-Fidelity PCR Master Mix (New England Biolabs). .. The mixed PCR products were purified, and sequencing libraries were generated using Ion Plus Fragment Library Kit 48 reactions (Thermo Scientific) following the manufacturer's protocol. ..

    Article Title: Porcine intestinal antimicrobial peptide as an in-feed antibiotic alternative improves intestinal digestion and immunity by shaping the gut microbiota in weaned piglets.
    Article Snippet: Antibiotic resistance of pathogens, which is caused by the abuse of in-feed antibiotics, threatens the sustainable development of livestock production.. The present study aimed to investigate the efficiency of porcine intestinal antimicrobial peptide (PIAP) as an alternative to in-feed antibiotics in terms of growth performance, intestinal morphology, digestive enzymes and immunity, and microbiota community of the post-weaning piglets.. A total of 204 piglets (Duroc Landrace Yorkshire, weaned at 28 d age) with a similar body weight of 7.97 ± 1.04 kg were randomly allocated to 4 groups (51 piglets per group): (1) control group: basal diet; (2) AB group: antibiotic, basal diet þ chlortetracycline (1000 mg/kg from d 1 to 24; 500 mg/kg from d 25 to 37); (3) P1 group: basal diet þ a relatively low dose of PIAP (400 mg/kg from d 1 to 24; 300 mg/kg from d 25 to 37); (4) P2 group, basal diet þ a relatively high dose of PIAP (600 mg/kg from d 1 to 24; 500 mg/kg from d 25 to 37).

    Construct:

    Article Title: Bioaccumulation, Ecotoxicity, and Microbial Responses in Hoplobatrachus rugulosus Tadpoles Following Co-Exposure to Imidacloprid and Microplastics.
    Article Snippet: .. Libraries were constructed using Ion Plus Fragment Library Kit 48 reactions (Thermo Fisher Scientific, Waltham, MA, USA) according to the standard protocols and were sequenced on an Ion S5TM XL platform. ..

    Article Title: Effects of Coumarin on Rhizosphere Microbiome and Metabolome of Lolium multiflorum
    Article Snippet: The purification of the PCR product was performed using a GeneJET gel kit (Thermo Fisher Scientific, Waltham, MA, USA). .. The Ion Plus Fragment Library Kit 48 reactions (Thermo Fisher Scientific, Waltham, MA, USA) was used to construct the library, and sequencing was performed on an Ion S5TMXL platform. ..

    Article Title: Application of fluoride disturbs plaque microecology and promotes remineralization of enamel initial caries
    Article Snippet: .. The library was constructed using an Ion Plus Fragment Library Kit 48 Reactions (Thermo Fisher, Germany). .. After the library was quantified by a Qubit Fluorometer (Thermo Scientific, Germany), the Ion S5 TM XL platform was used for sequencing to obtain the V4 variable-region base sequence information of the 16S rRNA.

    Polymerase Chain Reaction:

    Article Title: Insights into freshwater ciliate diversity through high throughput DNA metabarcoding
    Article Snippet: All PCR reactions were carried out with Phusion ® High-Fidelity PCR Master Mix (New England Biolabs). .. The mixed PCR products were purified, and sequencing libraries were generated using Ion Plus Fragment Library Kit 48 reactions (Thermo Scientific) following the manufacturer's protocol. ..

    Generated:

    Article Title: Insights into freshwater ciliate diversity through high throughput DNA metabarcoding
    Article Snippet: All PCR reactions were carried out with Phusion ® High-Fidelity PCR Master Mix (New England Biolabs). .. The mixed PCR products were purified, and sequencing libraries were generated using Ion Plus Fragment Library Kit 48 reactions (Thermo Scientific) following the manufacturer's protocol. ..

    Article Title: Porcine intestinal antimicrobial peptide as an in-feed antibiotic alternative improves intestinal digestion and immunity by shaping the gut microbiota in weaned piglets.
    Article Snippet: Antibiotic resistance of pathogens, which is caused by the abuse of in-feed antibiotics, threatens the sustainable development of livestock production.. The present study aimed to investigate the efficiency of porcine intestinal antimicrobial peptide (PIAP) as an alternative to in-feed antibiotics in terms of growth performance, intestinal morphology, digestive enzymes and immunity, and microbiota community of the post-weaning piglets.. A total of 204 piglets (Duroc Landrace Yorkshire, weaned at 28 d age) with a similar body weight of 7.97 ± 1.04 kg were randomly allocated to 4 groups (51 piglets per group): (1) control group: basal diet; (2) AB group: antibiotic, basal diet þ chlortetracycline (1000 mg/kg from d 1 to 24; 500 mg/kg from d 25 to 37); (3) P1 group: basal diet þ a relatively low dose of PIAP (400 mg/kg from d 1 to 24; 300 mg/kg from d 25 to 37); (4) P2 group, basal diet þ a relatively high dose of PIAP (600 mg/kg from d 1 to 24; 500 mg/kg from d 25 to 37).

    Electrophoresis:

    Article Title: Porcine intestinal antimicrobial peptide as an in-feed antibiotic alternative improves intestinal digestion and immunity by shaping the gut microbiota in weaned piglets.
    Article Snippet: Antibiotic resistance of pathogens, which is caused by the abuse of in-feed antibiotics, threatens the sustainable development of livestock production.. The present study aimed to investigate the efficiency of porcine intestinal antimicrobial peptide (PIAP) as an alternative to in-feed antibiotics in terms of growth performance, intestinal morphology, digestive enzymes and immunity, and microbiota community of the post-weaning piglets.. A total of 204 piglets (Duroc Landrace Yorkshire, weaned at 28 d age) with a similar body weight of 7.97 ± 1.04 kg were randomly allocated to 4 groups (51 piglets per group): (1) control group: basal diet; (2) AB group: antibiotic, basal diet þ chlortetracycline (1000 mg/kg from d 1 to 24; 500 mg/kg from d 25 to 37); (3) P1 group: basal diet þ a relatively low dose of PIAP (400 mg/kg from d 1 to 24; 300 mg/kg from d 25 to 37); (4) P2 group, basal diet þ a relatively high dose of PIAP (600 mg/kg from d 1 to 24; 500 mg/kg from d 25 to 37).

    Agarose Gel Electrophoresis:

    Article Title: Porcine intestinal antimicrobial peptide as an in-feed antibiotic alternative improves intestinal digestion and immunity by shaping the gut microbiota in weaned piglets.
    Article Snippet: Antibiotic resistance of pathogens, which is caused by the abuse of in-feed antibiotics, threatens the sustainable development of livestock production.. The present study aimed to investigate the efficiency of porcine intestinal antimicrobial peptide (PIAP) as an alternative to in-feed antibiotics in terms of growth performance, intestinal morphology, digestive enzymes and immunity, and microbiota community of the post-weaning piglets.. A total of 204 piglets (Duroc Landrace Yorkshire, weaned at 28 d age) with a similar body weight of 7.97 ± 1.04 kg were randomly allocated to 4 groups (51 piglets per group): (1) control group: basal diet; (2) AB group: antibiotic, basal diet þ chlortetracycline (1000 mg/kg from d 1 to 24; 500 mg/kg from d 25 to 37); (3) P1 group: basal diet þ a relatively low dose of PIAP (400 mg/kg from d 1 to 24; 300 mg/kg from d 25 to 37); (4) P2 group, basal diet þ a relatively high dose of PIAP (600 mg/kg from d 1 to 24; 500 mg/kg from d 25 to 37).



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